马铃薯叶肉细胞原生质体培养

Protoplast Culture of Potato's Mesophyll Cell

摘要:

马铃薯叶肉细胞原生质体培养后, 再生细胞形成细胞团和愈伤组织。愈伤组织经MS+2,4D 1.0mg/L+NAA 1.0mg/L+ZT 0.5mg/L+蔗糖2%继代后, 移至分化培养基MS+6-BA 1.0mg/L+NAA 0.1mg/L+ZT 1.0mg/L+AC 2g/L+蔗糖2%+甘露醇1%上分化效果较好。小植株在培养基MS+NAA 1.0mg/L上形成完整植株。

Abstract:

The mesophyll cell protoplasts of potato could divide from cell colonies and callus after cultured.The callosity was subcultured on the stock culture of MS+2,4-D 1.0 mg/L+NAA 1.0 mg/L+ZT 0.5 mg/L+sucrose 2%, differentiated on the medium of MS+ZT 1.0 mg/L+6-BA 1.0 mg/L+NAA 0.1 mg/L+AC 2 g/L+sucrose 2%+mannitol 1%.The plantlet formed whole plant on the medium of MS+NAA 1.0 mg/L.