大豆连作土壤中化感物质浸提剂的生物筛选

Bioassays with Extraction of Allelochemicals in Soybean Soil on Continuous Cropping

摘要:

通过大豆的正茬和重茬土壤水提液、磷酸缓冲液(pH=60)的乙醚萃取液和乙醇提取液对大豆和小麦种子萌发的研究表明:水和乙醇的正茬土壤提取液对大豆和小麦种子发芽无显著的抑制作用,它们的重茬土壤提取液对大豆种子发芽则有显著的抑制作用,其中水提液对小麦种子发芽无显著的抑制作用,高浓度的乙醇提取液则显著的抑制(正茬土壤)或促进(重茬土壤)小麦种子的发芽,正茬、重茬土壤磷酸缓冲液———乙醚萃取液对大豆和小麦种子的发芽均有显著的抑制作用。根据大豆和小麦的生产实际情况,我们认为纯水是进一步深入研究大豆连作障碍中土壤化感物质行之有效的土壤提取剂。

Abstract:

The soil extracts with water, phosphoric buffer solution-ether and ethanol were prepared and their inflnences on soybean and wheat germination (SSG and WSG)were studied. The results showed that:Extracts with both water and ethanol of SSRC don’t make significantly inhibition on SSG and WSG, while that with water and ethanol of SSCC significantly inhibit SSG, the water extract in which don’t significantly inhibit WSG; ethanol extract significantly inhibit (on SSRC)or hasten (on SSCC) WSG. Not only SSG but also WSG is significantly inhibited by phosphoric buffer solution-ether extract on SSRC and SSCC. Combining experiment results with practice, We suggest that water is the best among three extracts, by which allelochemicals of SSRC and SSCC could be made a thorough study.