Abstract:Recently, we have developed a compound bio-fertilizer, a new type of inoculants, based on research of Rhizobia fertilizer. It contains many kinds of beneficial microorganisms, whose strains are screened rigorously. Except Psendomons and Silicate bacteria, Azotobacter is the chief among these strains. At the same time, the optimum and composite strains are identified in physiological and biochemical characters, and established their effective targets. As the result, about Silicate bacteria, soluble potassium increases 131.51% in 10 days on the no nitrogen culture medium which contains feldspar powder; about Psendomons, Zone of soluble phosphvous can be observed obviously on the agar flate which contains cecithin; and nitrogenase activity of Azotobacter is 2.05 μmol C2H4/strain·h. We explore the fermented conditions of strains and find the formula for mix fermented broth, also do effective determination in safety, poison lessness and store tolerance of bacteria fertilizer.