Abstract:Using young root of Hordeum brevisubulatum (Trin.) Link as explant, calli were induced on basal medium MS with different concentrations of 2,4-D. Results showed the best concentiation was 4.0 mg/L. Calli were cultured on media with low concentration of 2,4-D. After the low shoot regenerated frequency appeared on MS with 2,4-D 1.0 mg/L + 6-BA 0.1 mg/L, those calli took roots on MS with IAA 0.5 mg/L + 6-BA 2.0 mg/L + KT 1.0 mg/L + sucrose 20 g/L, at last the whole plant regenerated.