Hydrogen Peroxide enzyme activity of soil under soybean continuous cropping stress was investigated by experiments. The results showed that hydrogen peroxide enzyme activity trooped significantly and it was reduced by 21.4% in soybean following soybean cropping for three years. Hydrogen peroxide accumulate in soil and aggravate poison to soybean roots as hydrogen peroxide enzyme activity drop obviously. The varinties and amount of roots exudations will change because nutrient elements uptake is obstructed by hydrogen peroxide poison to roots. In turn, it will inhibit soil enzyme activities.Therefore we think this is a kind of allelopathy under monocropping stress and a main reason of biological barriers caused by soybean continuous cropping.