中国林蛙的生产发展、科研成就及其展望

Tissue Culture of Root and Plant Regenerated from Hordeum brevisubulatum (Trin.) Link

摘要:

本文对中国林蛙的生产情况、研究成果及应用等进行了综述，同时对将来生产管理与科研方向进行了深入探讨，旨在对中国林蛙的科研与管理提供参考作用。

Abstract:

Using young root of Hordeum brevisubulatum (Trin.) Link as explant, calli were induced on basal medium MS with different concentrations of 2,4-D. Results showed the best concentiation was 4.0 mg/L. Calli were cultured on media with low concentration of 2,4-D. After the low shoot regenerated frequency appeared on MS with 2,4-D 1.0 mg/L + 6-BA 0.1 mg/L, those calli took roots on MS with IAA 0.5 mg/L + 6-BA 2.0 mg/L + KT 1.0 mg/L + sucrose 20 g/L, at last the whole plant regenerated.