野大麦幼根的愈伤组织诱导及植株再生

Tissue Culture of Root and Plant Regenerated from Hordeum brevisubulatum (Trin.) Link

摘要:

以野大麦（Hordeumbrevisubulatum（Trin.）Link）的幼根为外植体，采用MS培养基附加不同浓度的2，4-D诱导愈伤组织，结果以2，4-D 4.0 mg/L为最佳。以较低浓度2，4-D培养基进行愈伤组织的继代。在MS培养基附加2，4-D 1.0 mg/L+6-BA 0.1 mg/L，以极低的频率分化出芽，再在附加IAA 0.5 mg/L+6-BA 2.0 mg/L+KT 1.0 mg/L+Sucrose 20g/L上的MS培养基上生根，最终再生成完整植株。

Abstract:

Using young root of Hordeum brevisubulatum (Trin.) Link as explant, calli were induced on basal medium MS with different concentrations of 2,4-D. Results showed the best concentiation was 4.0 mg/L. Calli were cultured on media with low concentration of 2,4-D. After the low shoot regenerated frequency appeared on MS with 2,4-D 1.0 mg/L + 6-BA 0.1 mg/L, those calli took roots on MS with IAA 0.5 mg/L + 6-BA 2.0 mg/L + KT 1.0 mg/L + sucrose 20 g/L, at last the whole plant regenerated.