Abstract:According to the natural critical photoperiod and temperature of 2963 soybean accessions of different evolutionary types in their origination locations, and therules in which the double comprehensive effect occured, when the accessions grow underthe natural temperature in Gongzhuling and 13.5 hour daylength, we determined the ecotypes of soybean in China,and pointed out the natural critical photoperiod and temperatureof 74 counties and cities located at different latitude and height,discussed the relationshipbetween comprehensive effect of light and temperature where there is no wild soybean andthe formation of community and the importance of studing the theory of the comprehensiveeffect of light and temperature.