Soybeans with cold resistance and susceptible germinated under low temperture. And survey of the content of free proline was done. The result showed that the free proline increased with the days increased in a low germinate temperature (10℃). The accumlative process of free proline is slow in cold reaistance polan. The accumulation of free proline in polan is less than that in A5 and C1640 which with no cold resistance