RAPD标记在大豆品种鉴定中的应用初探

Use of RAPD for Identification of Soybean Cultivars

摘要:

以十六烷基溴化铵(CTAB)选择性沉淀DNA的方法，提取大豆种子、新鲜叶片或冷冻干燥叶片的总DNA，所得DNA纯度好，降解少，分子量大，完全可以用于RAPD分析。本文就RAPD标记手段在大豆品种鉴定中的应用作一简报，仅OPA-04就能将供试的17份大豆品种区分开，表明RAPD用于种子纯度鉴定及品种鉴定是可行的。

Abstract:

Random amplified polymorphic DNA(RAPD) was investigated as a means for identification of soybean cultivars. Extraction of total DNA by CTAB from soybean half-seed and fresh leaves is pure, with little degradation and large M. W. All of the seventeen soybean materials could be disting uished with only OPA^-04, indicating that RAPD can be used for identification of seed purity and varieties