芦苇草DNA导入小麦抗盐性转化体的筛选

Reed DNA Introducing into Wheat and Salt-resistant Transformation Screening

摘要:

以鲁麦8号小麦为受体，开花授粉0.5h后导入芦苇草DNA 10穗，113朵花。D₀收获66粒，D₁实收39个单株。从D₂中选出16个变异株，出现比较明显变异性状的是无芒、粒色、株高、穗长、穗粒数、颖尖的锐、钝等，特别是变异后代的抗盐性。以上变异后代通过自然盐渍土、盐池、水培筛选，选育出了89D₂₅₂₂₁₃、89D₃₁₁₁₁₉等抗盐性新品系。从形态特征和性状上看，说明芦苇草DNA已进入到鲁麦8号受体细胞核内，并对受体基因组产生了影响，其后代得到充分表达。

Abstract:

The introduction was made between wheet cultivar Lumai 8 as receptor and reed. After half hour of pollination 113 flowers of 10 ears were used for the introduction. The plants of D₀ genenation produced 66 grains. Then 39 plants were obtained from D₁ genenation and 16 variant plants were selected from D₂ genenation. The evident variations were found in several phenotypes, including the awn property, grain coclor, plant height, ear leath, grain number of each ear and sharp or dull of grain husk point, especially salt resistance. The variation were screened from nature salt soil, salt pond , water pond planting in late generations 89D₂₅₂₂₁₃ and 89D₃₁₁₁₁₉ and other salt_resistant lines were selected.