种子发育过程中野生大豆(G.soja)种子蛋白组分代谢研究初报

PRELIMINARY REPORT OF PROTEIN ANABOLISM IN WILD SOYBEAN SEEDS DURING DEVELOPMENT STAGE OF SEEDS

摘要:

利用SDS聚丙烯酰胺凝胶电泳,对开花后12到28天野生大豆种子贮藏蛋白进行了分析,结果表明: 1. 在发育过程中,野生大豆种子贮藏蛋白的性质发生了变化,初期以小分子量蛋白为主,随着种子的发育,大分子的贮藏蛋白逐渐增加。2.各种组分的合成起始时间不同,7S组分的α′、α亚基在开花后14天开始合成, 18天达到合成盛期; 7S组分的β亚基和11S组分的酸性、碱性亚基在开花后20天左右合成,28天达到合成盛期。3. α′、α亚基在发育过程中的代谢速率不同。

Abstract:

Quantitative and qualitative protein changes in developing wild soybean seeds (G. soja) were studied from 12 to 28 days after flowering by SDS gel electrophoresis. The samples used in this experiment was collected from Jilin province (45°N) and grown in Pots. The flowering date was noted for each flower and calculated the days from flowerino to sampling date. SDS gel electrophoresis was performed according tg the method of Sun and Hill. It was showed that the α and α′ subunits of 7s fraction appeared at about 14 days after flowering and the β subunit appeared late than α′ and α ones. The acidic and basic subunits of 11s fraction appeared at about 20 days after flowering. The increased rates of α′ and α subunts was different.