The effect of the low temperature on the growth and yield of Soybean was studied with CV.Jilin No.3 plants of different development period which had been treatmented at 10 ℃ and in light 14 h./day within ten days in a climatron.the result showed that the period of flower bud differenliation (from branching to flowering) was the most sensitive to the lower temperature.If Soybean plants were exposured to lower temperature in this time, the yield would be reduced by 60.1—60.8%.