The fat content of 1598 wild soybeans (G. soja) from different regions of China was 9.94% on thd average and lower significantly than that of 1595 cultivated soybeans (G. max), which was averaged19. 05%. The results of fatty acid composition of 174 wild, semiwild (G. gracilis) and cultivated soybeans showed that the linolenic and palmitic acid content decreased, while the oleic acid content increased as the soybean evoluted from wild to cultivated type. The effect of photoperiod and day and night temperature on fatty acid composition was analyzed. Some special genotypes, such as the linolenic content as high as 23. 12%, the linoleic acid content as high as 61. 24%, the oleic acid content as high as 51.55%, and the palmitic acid content as high as 15.49, were discovered in Chinese wild soybean germplasm.