Based on the physiological races identified in our country, soil samples representing 3 races of SCN were collected to evaluate the resistance of soybean breeding lines. The results indicated that there were 10 lines resistant to race 3, among which 7 were resistant to both race 1 and 3, and 5 were resistant to both race 3 and 4, 3 were resistant to race 1, 3 and 4. The results also showed that Jilin No. 20 could be used as a susceptible check to replace Essex.