Abstract:To identify a suitable LED spectral composition for producing high-quality blueberry seedlings, fourmonth-old blueberry seedlings were exposed to six light treatments: CK(white light control), T1(50% red light + 50% blue light), T2(60% red light + 40% blue light), T3(70% red light + 30% blue light), T4(75% red light + 25% blue light) and T5(80% red light + 20% blue light). The effects of the different spectral compositions on the growth traits and photosynthetic characteristics of the blueberry seedlings were comprehensively evaluated. The results showed that T2 and T3 significantly increased plant dry weight and the seedling quality index. T1 and T2 significantly increased photosynthetic pigment content, while T2 significantly increased soluble sugar content and root activity. The net photosynthetic rate, stomatal conductance and transpiration rate under T2 and T3 were significantly higher than those under CK. Intercellular CO2 concentration decreased as the proportion of red light increased, but the values under T1, T2 and T3 were significantly higher than that under CK. T4 and T5 significantly increased water-use efficiency. The actual photochemical quantum yield and electron transport efficiency under T1 were significantly lower than those under the other red–blue light combinations, although neither differed significantly from CK. The non-photochemical quenching coefficients under T2 and T3 were significantly lower than that under CK. Overall, T2 enhanced photosynthetic capacity by increasing light-use efficiency, photosynthetic pigment content and gas-exchange parameters, thereby promoting the accumulation of soluble sugars and proteins and increasing seedling dry weight. Thus, the T2 treatment is beneficial for producing high-quality blue‐berry seedlings.