番茄SNARE蛋白家族基因鉴定及分析

Identification and Analysis of SNARE Protein Family Genes in Tomato

摘要:

真核细胞的内膜系统主要通过囊泡运输实现物质交换，其中，囊泡膜与靶膜融合是囊泡运输最关键的环节，主要由可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体(Soluble N-ethylmaleimide-sensitive factor attachment protein receptors，SNARE)蛋白家族介导。植物生长发育依赖细胞分裂和扩增，SNARE蛋白可通过介导囊泡融合参与这些过程并响应环境胁迫。为探究番茄SNARE蛋白家族基因的生物学功能，本文基于生物信息学分析对其进行系统分析，共鉴定出65个番茄SNARE蛋白家族基因。系统发育、基因结构和氨基酸保守基序分析表明，SNARE蛋白家族基因分为5个亚族，同一亚族的氨基酸基序种类相似，具有保守性。顺式作用元件分析表明，SNARE蛋白家族基因包含大量与生长发育、激素响应和胁迫响应相关的顺式作用元件。本研究通过整合多维度分析，明确了番茄SNARE家族的分类特征与潜在调控机制，为后续基因功能验证及逆境响应分子机制解析奠定了理论基础。

Abstract:

The endomembrane system of eukaryotic cells primarily mediates material exchange through vesicular transport, in which the fusion of vesicle membranes with target membranes is the most critical step and is mainly mediated by the soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNARE) protein family. Plant growth and development depend on cell division and expansion, and SNARE proteins participate in these processes by mediating vesicle fusion and responding to environmental stresses. To explore the biological functions of SNARE protein family genes in tomato, a systematic bioinformatics analysis was performed, and a total of 65 tomato SNARE protein family genes were identified. Phylogenetic analysis, gene structure analysis and conserved amino acid motif analysis indicated that the SNARE protein family genes could be classified into five subfamilies, with similar types of amino acid motifs and high conservation within each subfamily. Cis-acting element analysis showed that the SNARE protein family genes contain numerous cis-acting elements related to growth and development, hormone responses and stress responses. By integrating multidimensional analyses, this study clarified the classification characteristics and potential regulatory mechanisms of the tomato SNARE family, thereby laying a theoretical foundation for subsequent functional verification of genes and for elucidating the molecular mechanisms underlying stress responses.