灰翅麦茎蜂线粒体基因组结构分析

Structural Analysis of the Mitochondrial Genome of Cephus fumipennis

摘要:

采用Illumina NovaSeq 6000平台对灰翅麦茎蜂的线粒体基因组进行双末端测序，注释分析其基因组序列特征，利用最大似然法构建灰翅麦茎蜂系统发育树。结果表明，灰翅麦茎蜂线粒体基因组全长18 981 bp，包括13个蛋白质编码基因(Protein-Coding Genes,PCGs)、22个tRNA基因、2个rRNA基因和1个非编码区，线粒体基因之间存在基因重叠或基因间隔的现象，呈闭合双链环状DNA分子结构。线粒体基因组中A+T含量高达80.41%，G+C含量为19.59%，呈现明显的AT偏向性；13个蛋白质编码基因均以通用密码子ATN作为起始密码子；20个tRNA基因形成典型的三叶草式二级结构，trnR、trnF基因因缺失TΨC环未形成典型的三叶草式结构。本研究获得了灰翅麦茎蜂线粒体基因组，明确了其基因组结构，并在线粒体基因组学水平上探讨灰翅麦茎蜂系统发育关系。

Abstract:

The Illumina NovaSeq 6000 platform was used to perform double-end sequencing of the mitochondrial genome of Cephus fumipennis, and the characteristics of the genome sequence were annotated and analyzed. The maximum likelihood method was used to construct the phylogenetic tree of Cephus fumipennis. The complete mitochondrial genome was 18,981 bp in length, including 13 protein-coding genes(PCGs), 22 tRNA genes, 2 rRNA genes and 1 non-coding region. There were gene overlaps or gene intervals between mitochondrial genes, showing a closed double-stranded circular DNA molecular structure. The A+T content in the mitochondrial genome was as high as 80.41%, and the G+C content was 19.59 %, showing obvious AT bias. The 13 protein-coding genes all used the universal codon ATN as the starting codon; the 20 tRNA genes formed a typical cloverleaf secondary structure, and the trnR and trnF genes did not form a typical cloverleaf structure due to the deletion of the TΨC loop. In this study, the mitochondrial genome of Cephus fumipennis was obtained, the genome structure was clarified, and the phylogenetic relationship of Cephus fumipennis was discussed at the level of mitochondrial genomics, which provided support and reference for the follow-up study of the phylogenetic relationship and green prevention and control of pests.