Abstract:In order to study the effects of different ratios of Pleurotus ostreatus and Pleurotus eryngii spent mushroom substrates to chicken manure on the fermentation parameters of organic fertilizer, and to screen the appropriate composting formula, five treatments with different mass ratios of spent mushroom substrates to chicken manure were set up in the experiment. Pleurotus ostreatus(P) and Pleurotus eryngii(XB) spent mushroom substrates were mixed with chicken manure at mass ratios of 150:0(Treatment 1), 120:30(Treatment 2), 90:60 (Treatment 3), 60:90(Treatment 4), and 30:120(Treatment 5), followed by inoculation with microbial agents. The effects of different ratios on fermentation temperature, organic matter content, total nutrient(nitrogen, phosphorus, potassium) content, C/N ratio, electrical conductivity(EC), pH value, and seed germination index(GI) were analyzed. The results showed that except for P1 and XB1, the fermentation temperature of other treatments exceeded 55℃ and lasted for more than 5 days. After fermentation, the organic matter content and C/N ratio of spent mushroom substrates(Pleurotus ostreatus and Pleurotus eryngii) in different treatments decreased significantly, and the organic matter content of treatments with chicken manure addition was significantly lower than that of treatments without chicken manure. Meanwhile, the nutrient content of all treatments increased; the total nutrient content and EC of treatments with chicken manure addition were higher than those without chicken manure, and EC increased with the increase of chicken manure addition. Excessive addition of chicken manure would affect the seed germination index. Comprehensive analysis showed that Treatments P2 and XB2 could meet the requirements of NY/T 525-2021 "Organic Fertilizer" and achieve better maturity standards, i.e., the optimal fermentation scheme is to mix Pleurotus ostreatus and Pleurotus eryngii spent mushroom substrates with chicken manure at a mass ratio of 120:30. However, due to the high EC(4.9 mS/cm) of Treatment XB2, it should be mixed with soil or other substrates before use.