Abstract:To systematically evaluate the regulatory effects of organic fertilizers containing polyglutamic acid on chrysanthemum yield, quality and the soil environment, a field experiment was conducted in the chrysanthemum planting area of Zhai Town, Xintai City in 2024. Three fertilization treatments were established: conventional fertilization(CK), conventional fertilization combined with organic fertilizer, and conventional fertilization combined with polyglutamic acid-containing organic fertilizer. The chrysanthemum yield and quality, the population sizes of soil bacteria, fungi, and actinomycetes, as well as soil enzyme activities under different treatments were systematically compared. The results showed that the application of polyglutamic acid-containing organic fertilizer not only significantly increased chrysanthemum yield and quality but also exerted a positive regulatory effect on the soil environment. Compared with the control(CK), the number of flowers per plant increased by 8.13%, the weight of 100 fresh flowers increased by 4.49%, and the yield increased by 12.58%. In terms of quality indicators, the total flavonoid content increased by 9.15%, chlorogenic acid content by 13.10%, total sugar content by 10.82%, and total amino acid content by 20.70%. The average population sizes of soil bacteria, fungi, and actinomycetes increased by 102.70%, 109.79%, and 44.81%, respectively. Soil urease activity increased by 15.69%, catalase activity by 15.13%, sucrase activity by 23.27%, and acid phosphatase activity by 19.60%. The application of polyglutamic acid-containing organic fertilizer as base fertilizer not only significantly improved chrysanthemum yield and quality but also effectively enhanced soil enzyme activities and microbial biomass.