Abstract:In order to clarify the effect of microbial organic fertilizer on soil health, the effects of four treatments—fallow land(no tillage and no fertilization), no fertilization, single application of chemical fertilizer, and combined application of 20% microbial organic fertilizer with 80% chemical fertilizer-on the physicochemical properties of hemp rhizosphere soil were studied. The results showed that compared with the other three treatments, the combined application of 20% microbial organic fertilizer with 80% chemical fertilizer increased the total porosity, capillary porosity, non-capillary porosity, and water content of hemp rhizosphere soil by 5.67%-23.60%, while the soil pH value and bulk density decreased by 1.51%-12.32%. This combined application also increased the contents of cation exchange capacity(CEC), organic matter, total nitrogen, total phosphorus, total potassium, available phosphorus, available potassium, and alkaline hydrolyzed nitrogen in hemp rhizosphere soil by1.88%-97.91%. Additionally, it increased the activities of urease, sucrase, acid phosphatase, and catalase, as well as the contents of total glomalin and easily extractable glomalin in hemp rhizosphere soil by 8.70%-270.00%. In conclusion, the combined application of microbial organic fertilizer effectively improved the physical properties of hemp rhizosphere soil, enhanced soil nutrients, enzyme activities, and glomalin content, and lowered soil pH value and bulk density. This study has laid a theoretical foundation for the restoration of black soil health.