Abstract:To achieve the goal of reducing chemical fertilizer application rate while improving its efficiency in farmland and increasing crop yields, a comparative study on soil fertility and yield was conducted in wheat-maize rotation fields by partially substituting compound fertilizer with organic fertilizer or microbial fertilizer. The results showed that partial replacement of compound fertilizer with organic fertilizer or microbial fertilizer for nutrient management in wheat-maize rotation fields can effectively enhance soil enzyme activity and soil fertility indicators. Wheat yield was positively correlated with soil total nitrogen content, available nitrogen content, available phosphorus content, organic matter content, urease activity, and alkaline phosphatase activity, but negatively correlated with soil invertase activity. In contrast, corn yield was negatively correlated with soil total nitrogen content, available nitrogen content, available phosphorus content, organic matter content, urease activity, alkaline phosphatase activity, and invertase activity. Specifically, it is suitable to apply organic fertilizer or microbial fertilizer during the winter wheat planting season, whereas compound fertilizer is more appropriate during the summer corn planting season. It is recommended to adopt a fertilization regime of 150 kg/ha microbial fertilizer combined with 450 kg/ha compound fertilizer as the optimal fertilization scheme for wheat-maize rotation fields. Compared with the treatment of applying only compound fertilizer, the total grain yield within one rotation cycle increased by 7.2%.