Abstract:The experiment was carried out with 'Yang Guifei Red Okra' as the test material, and the random block design method was employed to investigate the effects of four different volume ratios of wheat straw ash, coconut coir, vermiculite and perlite on the morphological index, photosynthetic index, vigor index, root activity and chlorophyll content of red okra seedlings, aiming to screen out the optimal seedling substrate for red okra. The results showed that the growth of the seedlings of red okra was significantly affected by the four different volume ratios of seedling substrate. The C treatment(wheat straw ash∶coconut coir∶vermiculite∶perlite=5∶2∶2∶1)had the best comprehensive performance, with leaf area of 66.32 cm2, vigor index of 0.77, stomatal conductance of 0.61 mol/(cm2·s), net photosynthetic rate of 26.14 μmol/(m2·s), transpiration rate of 4.97 mmol/(m2·m), and root activity and chlorophyll content were significantly higher than those of A, B, and D treatments. In conclusion, the C treatment can be used as the seedling substrate for red okra to cultivate robust seedlings and facilitate yield improvement.