产糖化酶的类芽孢杆菌S3菌株的多相分类鉴定

Polyphasic Taxonomic Identification of Paenibacillus Strain S3 Producing Saccharifying Enzyme

摘要:

本研究从甘薯种植地、淀粉厂厂区及下水道等有机质丰富的地方采取土样,从中分离产糖化酶细菌。其中一株命名为S3的分离菌株,根据形态特征观察、生理生化性状检测、全细胞脂肪酸成分分析、16S rDNA序列分析DNA-DNA杂交等鉴定实验结果表明属于类芽孢杆菌属(Paenibacillus)。根据16S rDNA序列构建的系统发育树,与S3进化距离相近的类芽孢杆菌菌种分别为:P. curdlanolyticus、P. kobensis和Paenibacillus sp. PALXIL08。但是,S3菌株在生理生化特征及细胞脂肪酸成分含量等都与这3种菌有所不同;DNA-DNA杂交实验表明S3和这3种菌的同源性分别为37.8%、21.7%和29.4%。因此,S3菌株可能是类芽孢杆菌的一个新种。

Abstract:

In this study, soil samples were collected from cropland, starch plant and sewerage respectively. An isolate named S3 was identified as Paenibacillus genus on the basis of physiological and biochemical characterization,16S rDNA sequencing, whole-cell fatty acid composition analysis and DNA-DNA hybridization. According to the phylogenetic tree constructed by 16S rDNA sequence, the phylogenetic distances of Paenibacillus like strains close to S3 were P. curdlanolyticus, P. kobensis and Paenibacillus sp. PALXIL08. The results of DNA-DNA hybridization showed that the homology of S3 and the three strains was 37.8%, 21.7% and 29.4%, respectively. Thus isolate S3 may represent a novel species of Paenibacillus.