Powdery mildew is a fungal disease in soybean production. It usually occurs in the environment with cool,high humidity and large temperature difference of day and night, and causes yield loss. In this study, 331 cultivated soybean accession mainly come from Northeast China were evaluated for the resistance to powdery mildew in greenhouse, using artificial supplementary inoculation with powdery mildew spores. The results showed that 85 accessions were highly resistant, accounting for 25.7%, and 83 accessions were highly susceptible, accounting for 25.1%.The proportions of high resistant germplasm were different from provinces, Liaoning > Jilin > Heilongjiang. The proportion of high resistant germplasm in land races was higher than that in improved varieties(lines). The disease index is significantly negatively related to growth period. Based on the results of disease resistance and agronomic characters, 12 highly resistant soybean varieties(lines) were proposed, which could be used resistant parents for the soybean breeding program in northeast China.