木聚糖酶改性红松松仁膜衣膳食纤维工艺优化及结构分析

Optimizing the Preparation Process of Dietary Fiber of Pinus Koraiensis Nut Coated-Film Modified by Xylanase and Analysis of Its Structure

摘要:

本试验以红松松仁膜衣为原料,对其不溶性膳食纤维进行木聚糖酶改性。采用响应面法优化改性工艺,研究各自变量对改性后松仁膜衣可溶性膳食纤维(Solubler Dietary Fiber of Pinus koraiensis nut coated-film,P-SDF)得率的影响,确定最优条件为:pH5.6、加酶量5 057 U/g、酶解时间75 min,改性后P-SDF得率达13.0%;通过扫描电子显微镜、红外光谱和X-射线衍射检测改性前后P-SDF,结果表明:改性后P-SDF的表面孔隙增加、疏松多孔、化学官能团变化较大、结晶区被破坏,可预测其理化功能性质得到改善。木聚糖酶改性是一种适合松仁膜衣膳食纤维改性的优良方法。

Abstract:

Pinus Koraiensis nut coated-film was used as raw material in this experiment, and its IDF was modified by xylanase. Response surface analysis was used to optimize the modification process to study interaction effect of variables on the yield of modified P-SDF. The optimal conditions were determined as follows: the pH was 5.6, the enzyme dosage was 5 057 U/g, and the enzymatic hydrolysis time was 75 min, and the yield of modified P-SDF was 13.0%. The structure of modified P-SDF was detected by scanning electron microscope, infrared spectroscopy and X-ray diffraction. The results showed that the surface pores of modified P-SDF increased, the chemical functional groups were greatly changed, the crystallization zone was destroyed, so its physical and chemical functional properties can be predicted to be improved. Xylanase modification is an excellent modification method on dietary fiber of Pinus Koraiensis nut coated-film.