玉米自交系I18人工接种大斑病菌的mRNA表达分析

Analysis of mRNA Expression of Maize Inbred Line I18 after Artificial Inoculating with Setosphaeria Turcica

摘要:

本研究对高感玉米自交系I18人工接种大斑病菌,经叶片表型鉴定,叶片病斑呈现增多且扩大的趋势。结果表明,对照与处理检测的表达基因与新基因数目不同,新基因占表达基因比例范围是11.95%～12.87%。自交系I18处理1,处理2、处理3共检测到1 401个共表达基因,上调基因926个,下调基因429个。从韦恩图可以看出,玉米接种大斑病后的基因共表达以上调为主,呈现被诱导激活的趋势,小部分基因表达受到抑制。随着大斑病侵染时间的增加,上调的基因数目呈现先增加后降低的趋势,下调的基因数目呈现升高的趋势,通过使用BLAST软件,对8大数据库进行序列比对,发掘1个下调的新基因,5个上调基因。经GO基因库进行功能注释,本试验检索检测到33 370个基因,分类得到32 048个注释基因,注释率达到96%。

Abstract:

A high-sensitivity maize inbred line I18 was artificially inoculated with northern corn leaf blight, and theleaf phenotype identification showed that the leaf macular degeneration increased and expanded. The results showedthat the number of the expressed genes was different from that of the new genes, and the proportion of new genes inthe expressed genes ranged from 11.95% to 12.87%. A total of 1 401 coexpressed genes, 926 up-regulated genesand 429 down-regulated genes were detected in inbred lines I18 and treatment 1, 2 and 3. As can be seen from theVenn diagram, the gene co-expression of corn after inoculation with macular disease was dominated by the aboveregulation, showing a trend of induced activation, and a small part of gene expression was inhibited. As the infectiontime of macular disease increased, the number of up-regulated genes increased at first and then decreased, and thenumber of down-regulated genes showed a trend of increase. The sequence alignment of 8 major databases was con-ducted by blast software, one down-regulated new gene and five up-regulated genes were founded. After functionalannotation by GO analyzed that 33 370 genes were retrieved and detected, 32 048 annotated genes were obtained byclassification, with the annotation rate reaching 96%.