Abstract:In order to study large-scale reproduction technology of trichogramma japonicum with rice moth eggs as intermediate host. a comparison experiment was conducted different proportion of female trichogramma to host eggs and time for inoculation, The results showed that proportion of female trichogramma to host eggs and time for inoculation had a great effect on the percentage of parasitism, and had no obvious effect on the adult emergence rate not the effect on the percentage of the female wasp. The percentage of parasitism were 91.22%, 90.49% and 84.71% respectively when proportions of trichogramma to eggs were 1∶10, 1∶15, 1∶20. The percentage of parasitism were relatively high when proportion of female Trichogramma to host eggs and time for inoculation were 1∶10 and 24-36 h, 1∶15 and 36-48 h, 1∶20 and 48-60 h. We should adopt the proportion of female trichogramma to host eggs as 1∶10 and time for inoculation is 24 h in mass production of Trichogramma japonicum species, which can improve the adult emergence rate of Trichogramma species. The ratio of bee egg to bee egg should be 1∶20 for 48 h in mass production in the factory.