花生吸水膨胀期耐低温性状QTL定位

Mapping of QTL for Low Temperature Tolerance Trait during Peanut Seed Imbibition

摘要:

本文目的是为我国花生耐低温育种中优良亲本的选择和分子标记辅助育种提供基本信息和材料基础。以徐花13中间型大粒花生为母本,中花6号珍珠豆型中粒花生为父本,衍生的187个家系的RIL群体通过SSR技术检测RIL群体多样性,构建了一张含325个标记的连锁图,总长度1 493.79 c M,标记间平均距离为4.60 cM,为遗传连锁图谱进一步饱和奠定基础。进行RIL群体耐低温表型鉴定,采用QTL IciMapping 3.0软件完备区间作图法进行QTLs定位和效应估计,共检测出10个与耐低温相关的QTL,其中主效QTL可以为耐低温标记辅助育种。鉴定出的耐低温家系可以为耐低温育种提供材料基础。

Abstract:

This paper provided basic for low temperature resistant breeding and molecular marker assisted breeding.187 recombinant inbred lines（RIL）, derived from a cross between two peanut cultivars（Xuhua 13×Zhonghua 6）,were used as mapping population. Finally, a genetic linkage map consisting of 325 SSR loci in 20 linkage groups and covering 1 493.79 cM with an average distance of 4.60 cM was constructed, which laid a good foundation for molecular genetic map construction. QTL mapping of the traits related to pod was conducted by QTL IciMapping 3.0. A total of 10 QTL were detected in the two environments, which laid a good foundation for molecular genetic map construction and the molecular markers linked closely to the major QTL could be used in MAS breeding of peanut with low temperature resistance. Low temperature resistant parentage may be basic germplasm resources in low temperature resistance peanut breeding.