Abstract:Matsutake is a rare edible fungus. It has received widespread attention for its anticancer, antitumor, antiviral and improve immunity. On the other hand, matsutake is the mycorrhizal fungi of pine, oak trees and other exogenous, which make it sparse in quantity, therefore it is known as "Spawn King." Aiming at matsutake, through the designing of single factor test and orthogonal test, the optimum extraction conditions of active peptides of Tricholoma matsutake was found in the paper. With the ratio of material to water 1:12, water to alcohol 1:2 and under the pH 9 condition, 10 h of the extraction at temperature of 28℃, more content of polypeptide could be extracted.