根癌农杆菌介导花生遗传转化体系的建立

Establishment of Agrobacterium-mediated Transformation System in peanut

摘要:

通过农杆菌介导法以花生下胚轴为外植体转化花生品种四粒红。经过共培养、抑菌和筛选培养,在含有0.8 mg/L Basta的培养基获得67株抗性再生植株。经PCR检测,获得5株阳性植株,bar试纸条检测至少有2株转基因植株为阳性。该体系操作简易、高效,可作为利用基因工程对花生进行研究和遗传改良的一种技术手段。

Abstract:

Agrobacterium-mediated transformation was performed in the peanut variet‘y Silihong’using hypocotyl as explants.After co-culture,bacteria restraining and selection,sixty-seven resistant regenerated plants were obtained on the medium with 0.8mg/L Basta.Five PCR-positive plants were tested by Quick-Strip Kit and at least two of them showed positive.The results showed that this protocol was an easy and high efficient,and will provide a practical method for the further genetic improvement of peanut.