放线菌769防治水稻稻瘟病的发酵条件研究

Studies on Fermentation Conditions of ‘Actinomycetes 769’ against Magnaporthe grisea

摘要:

对放线菌769防治水稻稻瘟病的最适培养基成分和发酵条件进行研究。结果表明,F培养基最适合该菌株的发酵培养,在此基础上对碳、氮源和无机盐进行了优化,通过正交试验确定了菌株769的最优发酵培养基配方为:黄豆豆粉2.0%、玉米粉1.50%、蔗糖1.0%、牛肉膏0.60%、碳酸钙0.30%、硫酸铵0.50%、氯化钠0.30%、硫酸镁0.10%、磷酸二氢钾0.02%、硫酸亚铁0.01%。通过对最佳发酵培养基初始pH值、接种量、摇瓶装液量、摇床转速等发酵条件进行正交试验设计,确定摇瓶最佳发酵条件组合为:种子液菌龄28 h,接种量4%,发酵时间72 h,培养基初始pH 6.72,培养基装量70 mL/250 mL三角瓶,发酵温度28℃,摇床转速180 r/min。

Abstract:

The fermentation medium and conditions of ’Actinomycetes 769’ against Magnaporthe grisea were stu died.The results indicated that the most appropriate fermentation medium was medium F.Furthermore,the carbon source,nitrogen source and mineral salts were optimized by Orthogonal experiment.The results showed that the optimum combination of medium and the optimal component were 2.0% soybean powder,1.5% corn powder,1.0% sucrose,0.60% beef extract,0.3% CaCO₃,0.5%（NH₄）₂SO₄,0.3% NaCl,0.1% MgSO₄,0.02% KH₂PO₄ and 0.01% FeSO₄,respectively.In this research,the optimum fermentation condition to enhance antibiotic activities of ‘Actinomycetes 769’ were as follows: culture temperature 28℃,pH 6.72,and seed age 28 hours,inoculation volume 4%,70 ml liquid medium in 250 ml flake,180 romin-1,and 72 hours in shaker.