费菜无性系建立的研究

Studies on Establishment of the Asexual Line of Sedum aizoon

摘要:

对费菜的嫩茎进行了无性系建立和快速繁殖的研究。结果表明:MS+BA0.5 mg·L^-1+2,4-D1.2 mg·L^-1是诱导嫩茎形成愈伤组织的理想培养基;1/2 MS+NH₄H₂PO₄50 mg·L^-1+AgNO₃1.0 mg·L^-1+BA1.0 mg·L^-1+NAA0.1mg·L^-1是愈伤组织分化培养的理想培养基;1/2 MS+NH₄H₂PO₄50 mg.L-1+AgNO₃1.0 mg.L-1+BA1.0 mg·L^-1+NAA0.1mg·L^-1+GA₃1.0 mg·L^-1是费菜不定芽继代分化培养的理想培养基;以1/2 MS+NAA0.1 mg·L^-1+IAA0.3 mg·L^-1为生根培养基,变温、自然光是费菜试管苗生根培养的理想条件;试管苗扦插成活率为94.5%,移植成活的试管苗株型大而整齐、根系发达,当年可正常开花结实。

Abstract:

A asexual line and method of rapid propagation of Sedum aizoon were established for the young stem.The results showed that the best medium to induce the callus of Sedum aizoon was MS + 0.5 mg·L^-1 BA + 1.2 mg·L^-1 2,4-D.The best medium to induce differentiation of callus was 1/2 MS + 50 mg·L^-1NH₄H₂PO₄ + 1.0 mg·L^-1AgNO₃ + 1.0 mg·L^-1BA + 0.1 mg·L^-1NAA.The idea medium for differentiation and subculture of the adventitious bud was 1/2 MS + 50 mg·L^-1NH₄H₂PO₄ + 1.0 mg·L^-1 AgNO₃ + 1.0 mg·L^-1BA + 0.1 mg·L^-1NAA + 1.0 mg·L^-1GA₃.The optimum medium for rooting under the condition of heterotherm and natural light was 1/2 MS + 0.1 mg·L^-1NAA + 0.3 mg·L^-1 IAA.The rate of surviving was 94.5%.The seedling grew great and neatly with flourishing root and blooming and seeding in the year of transplanting.