A preliminary experiment to determine the effect of inoculation times on the parasitic rate, the eclosion rate, the number in a single host egg and sex rate of three strains of Trichogramma chilonis to tussah eggs was performed in the laboratory. The results were summarized as follows: much difference existed among three strains and TC strain was better than anyone else in different times of inoculation. It was identified that TC strain can be used in factory reproduction on a large scale.